Innovation
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Nexogen focuses on the development and commercialization of disruptive technologies that bring low cost, rapid detection platforms to point-of-care diagnostics in human and animal clinics.
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Microfluidics
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Three microfluidics platforms have been developed to enable integration of sample preparation, nucleic acid amplification, and fluorescence detection of DNA/RNA in instrument platforms targeting different sample throughput.
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The platforms integrate: (i) an efficient sample preparation based on combining chemical, enzymatic and mechanical lysis; (ii) a rapid DNA/RNA amplification including PCR and isothermal amplification reactions, and (iii) an accelerated electrophoretic accumulation of DNA/RNA in an array format.
Array Technologies
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Electrokinetically enhanced accumulation of DNA/RNA targets on an electrode array and/or on an array of well detection chambers
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Carbon Array Technology - low cost, screen printed arrays of electrodes accelerate target accumulation, so the hybridization to specific probes occur within minutes, compared to hours in passive microarrays
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Manufacturing technology is as simple as in the glucose sensors, using similar screen printed inks
Sample Preparation
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The proprietary DNA extraction method combines mechanical, enzymatic, and chemical lysis of nucleic acids
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DNA/RNA extraction efficiency is comparable or exceeds current reagents / kits on the market
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Sample preparation is completed within 6-8 minutes
Assays and Reagent Kits
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To promote our instrument/cartridge platforms worldwide, we develop assays and assay kits through collaborative ventures offered to partners interested in point-of-care DNA diagnostics
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The assays target point-of-use, decentralized and field application markets, as well as R&D and educational molecular biology laboratories
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One of our market niche interests is veterinary diagnostics of infectious diseases including pet clinics, high-end equine assays of interest, avian diseases, high consequence bovine, poultry, and swine diseases on farms
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Panel assays for: (i) hospital acquired infections; (ii) respiratory viral panels; and (iii) emerging traveller panels, are developed through partnerships
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Reagent kits are available for generic sample preparation protocols for extraction of DNA from various samples
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(see relevant papers published)